Blue pippin system

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August undefined, 2024

Web9 Apr 2024 · Large DNA fragments (> 8 to 10 kb) were recovered using a Blue Pippin system (Sage Science, Beverly, MA, USA). Next, genomic DNA libraries were prepared using a Ligation sequencing 1D kit (SQK-LSK109, ONT, Oxford, UK). Finally, DNA fragments were sequenced with ONT PromethION sequencer. WebThe fragment size (>10 kb) was selected by blue pippin system to prepare SMRTbell DNA template libraries. DNA sequencing was per- formed on the Paciﬁc Biosciences Sequel (PacBio, Menlo Park, CA, U.S.A.) by G enedenovo Biotechnology Co., Ltd. (Guangzhou, China). In all, 145,826 reads and 1,162,120,421 bp were obtained and the N

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WebMapper® XA Pulsed Field Electrophoresis System Before You Begin This procedure can be used to evaluate genomic DNA (gDNA) to ensure that it is of high quality and high ... Blue Pippin system 7. 50 kb Shear – Size Selected at 30 kb using Blue Pippin system Figure 5. Evaluation of gDNA quality by PFGE using a CHEF Mapper XA Syste m WebThe BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. With SDS … shreecem tradingview

BluePippin and Pippin HT DNA Technologies Core

WebBlue Pippin Fractionator and Covaris Ultrasonicator Explore Genomics facilities Antenna lab We have built an extensive antenna test range and electromagnetic characterisation facility, which now virtually covers the whole RF to … WebThere are three components to the BluePippin system: Instrument – The instrument includes epifluorescence detectors, an electrophoresis power supply, an electrode array, … Web3 Sep 2024 · Quality and quantity of total DNA were evaluated using a NanoDrop One UV–Vis spectrophotometer (ThermoFisher Scientific, Waltham, MA) and Qubit 3.0 Fluorometer (Invitrogen life Technologies, Carlsbad, CA), respectively. The Blue Pippin system (Sage Science, Beverly, MA) was used to retrieve large DNA fragments by gel … shree cements balance sheet

Long-read, whole-genome shotgun sequence data for five model …

Mic Real-Time PCR System - Labgene

WebThe Blue Pippin system from Sage Science enables targeted size selection. High-pass filtering kits for the Blue Pippin are beneficial for long-read sequencing, as they allow the … Web28 Sep 2024 · Nature Genetics - Haplotype-resolved assembly of a heterozygous diploid potato, RH89-039-16, and identification of deleterious mutations provide insights into the genome organization and breeding... shreecem nseWeb3 Feb 2024 · Libraries were size-selected using Blue Pippin system with 2% gel Blue Pippin Cassette (Sage Science, United States) with a range of 200–600 bp and their quality and quantity was assessed using Agilent DNA kit (Agilent technologies, United States). Libraries were pooled and sequenced using a NextSeq 500/550 High Output Kit v2.5 … shree cement swot analysis

"WebDescription. Internal price (Cornell and Cornell affiliates) External price. Full Service Sequencing, plasmid or PCR product, per sample, template premixed with sequencing primer. $4.30. $7.05. Special Conditions--dGTP addition, BAC sequencing, added to the single sample sequencing cost, per reaction. $5. " - Blue pippin system

Blue pippin system

WebCompleting runs in under 30 minutes is the new standard with Mic, not the exception. 5 point, 2x dilution series of Hepatitis B virus (HBV) cDNA template. Starting amount of 3E+06 copies (n = 4 each) Efficiency = 90% (standard curve method) ; R² = 0.99. Time to complete run (including melt) = 26 min. Web2 Sep 2024 · Blue and gold dots indicate significant downregulated and up-regulated genes, respectively. ... Pippin system (Sage Science, Beverly, MA, USA) to remove fragments less than 15 Kb was .

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Web1 Aug 2024 · The DNA for this library was prepared by performing a high-pass size-selection to collect DNA fragments >40 kb using a 0.75% agarose gel cassette and external U1 marker for the Blue Pippin system (Sage Science, Beverly, MA, USA) following manufacturer’s protocol, followed by concentration and cleaning with Ampure beads.

WebBehind the Tech: Pippin Prep and BluePippin sagescience 92 subscribers Subscribe 39 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from … WebLabtech

Web29 Mar 2024 · Libraries were prepared using the SMRT bell library prep protocol (Pacific Biosciences), followed by DNA size selection using the Blue Pippin system (Sage Science) to remove short insert templates. Subsequently, M. chitwoodi DNA was sequenced on PacBio Sequel RS II (Pacific Biosciences). Genomes were assembled using the SMRT … WebSage-Scientific Blue Pippin; Life Technologies E-Gel System; Diagenode Bioruptor Standard Sonicator; Gene Expression. Quantitative PCR (qPCR) Affymetrix GeneChip Workstation; Imaging. Typhoon 9410 GE Healthcare; Biorad Gel Doc EZ; Incubators/Shakers. Labnet Vortemp 56 incubator; Mcconnell Research Mini-grow 384 …

Webselection using the Blue Pippin system (Sage Science) to remove short insert templates. Subsequently, M. chitwoodi DNA was sequenced on PacBio Sequel RS II (Paci ﬁc Biosciences). Genomes were...

WebLibraries were size-selected using Blue Pippin system with 2% gel Blue Pippin Cassette (Sage Science, United States) with a range of 200–600 bp and their quality and quantity was assessed using Agilent DNA kit (Agilent technologies, United States). Libraries were pooled and sequenced using a NextSeq 500/550 High Output Kit v2.5 (300 cycles ... shree ceramicsWebThe BluePippin system uses dye-free, pre-cast gel cassettes to separate and extract desired DNA fragment size ranges from agarose. It enables size selection for fragment … We are a core facility committed to providing quality sequencing and … Discuss your projects with Kristin Mars, Field Applications Scientist Discover the … The Infinium platform from Illumina is an extremely high throughput SNP … The Bioinformatics Core at the UC Davis Genome Center has over a decade of … Research to develop the TILLING_by_Sequencing method using … The Center. The West Coast Metabolomics Center is a cluster center with four … Any Questions? Contact Us. Phone: (530) 754-9474 (we are not routeinly by the … The Perkin Elmer LabChip GX Touch HT is a “High-Throughput Bioanalyzer” … shree cements ltd share priceWeb6 Apr 2024 · Libraries were size selected via the Blue Pippin system (Sage Science) to isolate ~ 260 bp fragments. Libraries were verified on a High Sensitivity DNA chip using the 2100 Bioanalyzer (Agilent). Sequencing was performed by GenomeQuebec on a HiSeq4000 Illumina system by multiplexing 12 samples per flow cell and performing 100 bp paired … shree cem share priceWeb11 Nov 2014 · PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin … shree ceramic fibers private limitedWeb16 Jan 2024 · 314 – 414 bp using the Blue Pippin system. We predicted that there were 117,528 SLAF tags. distributed in the genome with 6.19% located in repetitive regions. To further evaluate the. ef ... shree ceramic fibers pvt. ltdWeb2 Sep 2024 · For each Nanopore library, the gDNA was size-selected (10–50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION … shree chaitanya iit test seriesWeb7 Jan 2024 · Sheared genomic DNA was treated for damage repair and end repair, adaptor ligation, and size selection with a Blue Pippin system (Sage Science) to create SMRTbell template libraries. The final libraries were sequenced on the PacBio Sequel platform (Pacific Biosciences, CA, USA) using Sequel Sequencing Kit 2.1 Bundle. ... shree chaitanya institute pune