WebNational Center for Biotechnology Information WebAbstract. A transformation scheme for Cryptococcus neoformans to yield high-frequency, integrative events was developed. Adenine auxotrophs from a clinical isolate of C. …
Multiple Applications of a Transient CRISPR-Cas9 Coupled …
WebJun 2, 2024 · Cryptococcus neoformans is a major etiological agent of fungal meningoencephalitis. The outcome of cryptococcosis depends on the complex interactions between the pathogenic fungus and host immunity. The understanding of how C. neoformans manipulates the host immune response through its pathogenic factors … WebCryptococcus neoformans, the most common cause of fungal meningitis, is a basidiomycete haploid budding yeast with a complete sexual cycle. Genome modification by homologous recombination is feasible using biolistic transformation and long homology arms, but the method is arduous and unreliable. Recently, multiple groups have reported … portland house pub sheffield
Novel biosynthetic pathway for sulfur amino acids in Cryptococcus …
WebJan 15, 2024 · Cryptococcus; selectable gene marker; electroporation; biolistic transformation; CRISPR- Cas9 technique 1. Introduction Genetic transformation plays an imperative role in our understanding of... WebJan 1, 2012 · Inducible promoters are invaluable tools for modulating gene expression (turning transcription on or off) and have been a key approach for ascertaining gene essentiality in Cryptococcus neoformans.Galactose-inducible promoters have been successfully used in Saccharomyces cerevisiae to manipulate heterologous gene … WebFeb 1, 2000 · Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle and well-developed genetic and molecular approaches. Two different transformation systems have been developed, and a number of genes have been disrupted by homologous recombination. portland house pitstone