WebOocytes were incubated in OR2 media, which consists of 82.5 m m NaCl, 2.5 m m KCl, 1 m m CaCl 2, 1 m m MgCl 2, 1 m m Na 2 HPO 4, 0.5 g/liter polyvinyl pyrrolidine, and 5 m m HEPES (pH 7.2), supplemented with penicillin and streptomycin (0.1 mg/ml) and 2 m m sodium pyruvate. Oocytes were then injected with 52 nl of cRNA mix for a final amount of ... WebAug 5, 1990 · Two novel facilitative glucose transporter-like cDNAs have been isolated from human small intestine and fetal skeletal muscle cDNA libraries by low stringency cross-hybridization with a fragment of the human erythrocyte/GLUT1 facilitative glucose transporter cDNA.

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WebCytochalasin D treatment Oocytes were incubated in CytD-containing OR2 medium for 2.5 h at room temperature. CytD (Sigma) was diluted to a final concentration of 4 μM. After incubation, the nuclei were isolated from the oocytes and fixed in 4% paraformaldehyde in PBS containing 0.2% Triton X-100. Web1 Supervisory Policy Manual OR-2 Operational Resilience V.1 - Consultation This module should be read in conjunction with the Introduction and with the Glossary, which contains … hopewell valley high school boys lacrosse

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