Phospho-smad2 ser465/467 /smad3 ser423/425

WebMANUFACTURER ITW Plexus 30 Endicott St. Danvers, Massachusetts 01923 Emergency telephone number (CHEMTREC): (800) 424-9300 Other Calls: (978) 777-1100 WebAug 18, 2024 · SIS3, a selective inhibitor of Smad3 ... p38 MAPK inhibitor) were from Tocris Biosciences (Bristol, UK). Antibodies of Jag1, phospho-Smad2 (Ser465/467), phospho-Smad3 (Ser423/425), phospho-ERK1/2 (Thr202/204), phospho-JNK (Thr183/Try185), phospho-p38 MAPK (Thr180/Tyr182), and myosin phosphatase target subunit 1 (MYPT1) …

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WebOct 31, 1997 · Phosphorylation sites on Smad2 were localized to a carboxyl-terminal fragment containing three serine residues at positions 464, 465, and 467. In this report, we show that TbetaRI specifically phosphorylates Smad2 on serines 465 and 467. Serine 464 is not a site of phosphorylation, but is important for efficient phosphorylation of Smad2. Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. This antibody also recognizes endogenous levels of Smad3 protein when phosphorylated Ser422 only or at both Ser423 and Ser425. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification sims 4 rirrier hair https://mbrcsi.com

Phosphor Smad3 Ser423 Bioz Ratings For Life-Science Research

WebApr 26, 2024 · Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Catalog Number: 8828. Clone Number: N/A. Info:Supplier Page View Product Specs. Browse Similar Products: SMAD2 Flow Cytometry Antibodies. Cells were stimulated with TGF-b in 0.5% FBS for 24 hours. Our research is focused on the SMAD pathway that regulates a diverse set of … WebThe total Smad2, Smad3, and Smad4 antibodies detect their respective targets at endogenous levels. Source/Purification: Phospho-specific monoclonal anti-bodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser465/467 of human Smad2 or Ser423/425 of human Smad3 protein. WebLysates were blotted using Purified Mouse Anti-Smad2 (pS465/pS467)/Smad3 (pS423/pS425) antibody (Clone O72-670; 2 µg/mL). Phosphorylated Smad2 and Smad3 were identified as ~60 kDa and ~52 kDa bands, respectively. The lower molecular weight band detected in mouse spleen lysates is endogenous mouse Ig light chain. rcgp midland faculty facebook

Phospho-Smad2 (Ser465/467) Antibody - Cell Signaling …

Category:Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, ZooMAb

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Phospho-smad2 ser465/467 /smad3 ser423/425

Phospho-Smad2 (Ser465/467) Antibody Cell Signaling

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Phospho-smad2 ser465/467 /smad3 ser423/425

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WebJun 19, 2024 · Smad2/3 activation was analyzed by the phosphorylation level at Ser465/467 in Smad2 and Ser423/425 in Smad3. In addition, Stat3 activation was analyzed by the phosphorylation level at Tyr705. (b ... WebResults: A total of 161 patients with NFPAs were identified; 59 (36.6%) had invasive NFPAs and 102 (63.4%) had noninvasive NFPAs. Protein levels of Smad3 and phospho-Smad3 (p …

WebOct 31, 1997 · Phosphorylation sites on Smad2 were localized to a carboxyl-terminal fragment containing three serine residues at positions 464, 465, and 467. In this report, … WebThe Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb Antibody has been validated for the following applications: Western Blot. Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit …

http://www.asbio.com.cn/shop/category/102-392/page/5 WebThe Phospho-SMAD2 (Ser465/467) kit enables the cell-based quantitative detection of SMAD2 phosphorylated on Ser465/467, as a readout of the TGFb pathway. See more All inclusive kit High sensitivity Rapid Low sample consumption Download the protocols Size Cat. number selected : - Quantity Request for quotation Prices may differ between countries.

WebPhospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D6G10) XP ® Rabbit mAb detects endogenous levels of phosporylated Smad2 and Smad3. Some reactivity is observed with …

WebThe O72-670 monoclonal antibody specifically binds to the Smad2 protein phosphorylated at the Ser465/467 sites and the Smad3 protein phosphorylated at the Ser423/425 sites. Smad2 and Smad3 are members of the Smad superfamily with observed molecular weights of 60 kDa and 52 kDa, respectively. rcgp monkeypoxrcgp minor surgery update how oftenWebAnti-phospho-Smad2 (Ser465/467), clone A5S ZooMAb, Cat. No. ZRB04953, is a recombinant Rabbit Monoclonal Antibody that detects Smad2 phosphorylated on serine 465/467 and has been tested forr use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. rcgp member ceremonyWebPhospho-SMAD3 (Ser423, Ser425) Antibody (44-246G) in ICC/IF. Immunofluorescence analysis of SMAD3 (pSpS423/425) was done on 70% confluent log phase HeLa cells treated with TGF-beta (20 ng/mL for 15 min) cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with ... rcgp minor surgery courseWebAnti-phospho-Smad2 (Ser465/467), clone A5S ZooMAb, Cat. No. ZRB04953, is a recombinant Rabbit Monoclonal Antibody that detects Smad2 phosphorylated on serine … rcgp minor surgery course glasgowWebAnti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, ... (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. sims 4 road to fame mod deutschWebJan 11, 2024 · The SMURF2Thr249phosphorylation level was low in human glioblastoma pathology specimens. Introduction of the SMURF2T249Amutant resulted in increased stemness and tumorigenicity of GSCs, recapitulating the SMURF2silencing. Moreover, the inactivation of SMURF2Thr249phosphorylation increases TGF-β receptor (TGFBR) protein … sims4 r o a c h b a b y e t e a r s