Phospholipidosis assay
WebeCiphr ® Cardio — iPS cell-derived cardiomyocytes and MEA. Structural cardiotoxicity assay — using 3D spontaneously beating microtissues. Combined hypertrophy and cardiotoxicity assay — using 3D spontaneously beating microtissues. Cardiotox screen — … WebMembrane permeability assays demonstrate that the alkaloids are biologically available, although fangchinoline showed lower permeability than tetrandrine. At high concentrations, the extract, alkaloid fractions, and pure alkaloids induced phospholipidosis in 293TAT cells and less so in VeroAT cells. Gene expression profiling during virus ...
Phospholipidosis assay
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WebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a leading cause of attrition during drug development. WebMar 24, 2024 · Drug-induced phospholipidosis: Human: ASCs: Matrigel: Expression of CYP450 enzyme (CYP3A4); ... (LoT) assay to analyze the hepatoxicity of 238 marketed drugs (32 negative controls and 206 reported DILI agents) and showed high predictive values of the LoT system with 88.7% sensitivity and 88.9% specificity.
Webcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a WebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of phospholipidosis is still unclear today, but it is known that phospholipidosis is commonly caused by the intake of a special group of drugs containing a hydrophobic ring/chain and a hydrophilic amine …
WebJun 22, 2024 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established … WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform.
WebWhen studying phospholipidosis/steatosis in vitro, IONTOX uses HepG2 cells in combination with LipidTox Red, as our primary model to discriminate between phospholipidosis …
WebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … chip haldemanWebFeb 1, 2006 · To investigate the role of phospholipidosis in antiviral activity in vitro, we tested 19 drugs for their induction of this effect in A549 cells using the well-established NBD-PE staining assay... gran torino console wiringWebPhospholipidosis (PL) is a phospholipid storage disorder, resulting in excessive accumulation of phospholipids in lysosomes of various tissue types [199, 200]. From:Progress in Medicinal Chemistry, 2011 Related terms: Amiodarone Patient Phospholipid Storage Disease Lysosome Storage Disease Niemann Pick Disease … chip haircutWebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high … chip halbauerWebPhospholipidosis (PLD) is a lysosomal storage disorder induced by compounds, notably cationic amphiphilic drugs, which although reversible interferes with cellular … gran torino characterizationWebJul 7, 2004 · Required concentrations for increased Nile Red binding were generally three-fold or more lower than the cytotoxic concentration determined by a resazurin reduction assay in the same cells. Qualitatively similar Nile Red binding results were obtained when primary canine or rat hepatocytes were used. chip guard whiteWebEchelon’s LPLA2 Inhibitor Screen is a high throughput assay designed to screen drugs abilitiy to inhibit LPLA2 activity in vitro, a potential predictor of drug-induced phospholipidosis. Sample Volume: 1 μL. Sample Number: 128 Samples (in triplicates)/384-well plate. Echelon’s LPLA2 Inhibitor Screen (K-7000I) is designed to assay a drug’s ... chip halbleiter